Polyclonal Antibody to Calreticulin (CALR) Homo sapiens (Human) Polyclonal antibody

CRT; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB486Hu02-Recombinant Calreticulin (CALR)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Calreticulin (CALR) Packages (Simulation)
  • Polyclonal Antibody to Calreticulin (CALR) Packages (Simulation)
  • Polyclonal Antibody to Calreticulin (CALR) DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CRT Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Calreticulin (CALR) DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human CRT Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Calreticulin (CALR) DAB staining on IHC-P;
    Samples: Human Small intestine Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human CRT Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB486Hu02.jpg Figure. Western Blot; Sample: Recombinant CALR, Human.
  • Polyclonal Antibody to Calreticulin (CALR) Western Blot; Sample: Mouse Cerebrum lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human CRT Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Calreticulin (CALR) Western Blot; Sample: Human HL60 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human CRT Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Calreticulin (CALR) Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CALR Polyclonal Antibody (Catalog PAB486Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CALR. It has been selected for its ability to recognize CALR in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Oxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of MelanocytesPubmed: 23771121
  • The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKCMdpi: Source
  • Amniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranesPubmed:26953684
  • Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes.pubmed:28152632
  • Investigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modelingDoi: 10.1016/j.talanta.2018.11.098
  • Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemiaPubmed:35171673

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