Polyclonal Antibody to Carbonic Anhydrase I (CA1) Mus musculus (Mouse) Polyclonal antibody

CA-I; CAI; Car1; CAB; Carbonic anhydrase B; Carbonate dehydratase I

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Carbonic Anhydrase I (CA1) Packages (Simulation)
  • Polyclonal Antibody to Carbonic Anhydrase I (CA1) Packages (Simulation)
  • Polyclonal Antibody to Carbonic Anhydrase I (CA1) DAB staining on IHC-P; Sample: Mouse Thymus Tissue Primary Ab: 10µg/ml Rabbit Anti-Mouse CA1 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Carbonic Anhydrase I (CA1) DAB staining on IHC-P;
    Sample: Mouse Spleen Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse CA1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Carbonic Anhydrase I (CA1) DAB staining on IHC-P;
    Sample: Mouse Lymph node Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse CA1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA875Mu01.jpg Figure. Western Blot; Sample: Recombinant CA1, Mouse.
  • Polyclonal Antibody to Carbonic Anhydrase I (CA1) Western Blot; Samples: Lane1: Rat Cerebrum lysate; Lane2: Rat Spleen lysate; Lane3: Mouse Spleen lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Mouse CA1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CA1. It has been selected for its ability to recognize CA1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Giveaways

Citations

  • Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical SignificancePubMed: PMC3754973
  • Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patientsPubmed:26680509
  • Serum Carbonic Anhydrase 1 is a Biomarker for Diagnosis of Human Schistosoma mansoni Infection.pubmed:28500821
  • Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosisS0021967317311858

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