Polyclonal Antibody to Caspase 14 (CASP14)

Caspase 14 Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 14

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 95.00 US$ 221.00 US$ 316.00 US$ 790.00 US$ 3,160.00
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Overview

Properties

Product No.PAA457Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryApoptosis

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA457Hu01-Recombinant Caspase 14 (CASP14)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

FITC staining on IF; Samples: Human U2OS cell; Primary Ab: 20μg/ml Rabbit Anti-Human CASP14 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)

Western Blot; Sample: Porcine Skin lysate Primary Ab: 0.2μg/ml Rabbit Anti-Human CASP14 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Mouse Kidney lysate; Lane2: Rat Skin lysate
Primary Ab: 2μg/ml Rabbit Anti-Human CASP14 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CASP14. It has been selected for its ability to recognize CASP14 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

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Citations

Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitisPubmed:25315296
Luteolin induces caspase-14-mediated terminal differentiation in human epidermal keratinocytesPubMed: 26427706
Enhanced Induction of Apoptosis in HaCaT Cells by Luteolin Encapsulated in PEGylated Liposomes—Role of Caspase-3/Caspase-14Pubmed: 30390204