Polyclonal Antibody to Caspase 8 (CASP8) Rattus norvegicus (Rat) Polyclonal antibody

CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA853Ra01-Recombinant Caspase 8 (CASP8)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Caspase 8 (CASP8) Packages (Simulation)
  • Polyclonal Antibody to Caspase 8 (CASP8) Packages (Simulation)
  • Polyclonal Antibody to Caspase 8 (CASP8) DAB staining on IHC-P; Samples: Rat Thymus Tissue; Primary Ab: 10μg/ml Rabbit Anti-Rat CASP8 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 8 (CASP8) DAB staining on IHC-P;
    Samples: Rat Colon Tissue;
    Primary Ab: 10μg/ml Rabbit Anti-Rat CASP8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 8 (CASP8) DAB staining on IHC-P;
    Sample: Rat Colon Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat CASP8 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 8 (CASP8) DAB staining on IHC-P;
    Sample: Rat Thymus Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat CASP8 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA853Ra01.jpg Western Blot; Sample: 3T3-L1 cell lysate Primary Ab: 1μg/ml Rabbit Anti-Rat CASP8 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 8 (CASP8) Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Rat Cerebrum lysate; Lane3: Rat Placenta lysate; Lane4: Mouse Liver lysate; Lane5: Mouse Cerebrum lysate; Lane6: Mouse Lung lysate; Lane7: Porcine Spleen lysate; Lane8: Porcine Lung lysate; Lane9: Porcine Liver lysate; Lane10: Raji cell lysate; Lane11: RAW264.7 cell lysate
    Primary Ab: 0.2μg/ml Rabbit Anti-Rat CASP8 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CASP8. It has been selected for its ability to recognize CASP8 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial PerturbationsPubmed:25502462
  • Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cellsPubmed:29555410
  • Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathwayPubmed:29266336
  • Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino micePubmed: 31087429
  • Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in ratsPubmed: 31421311
  • Tranilast attenuates methotrexate‐induced renal and hepatic toxicities: Role of apoptosis‐induced tissue proliferationPubmed: 32045101
  • Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in ratsPubmed: 32259601
  • Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cellsPubmed: 33206337
  • Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signalingPubmed: 33199237
  • Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathwaysPubmed: 32942000
  • Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of …Pubmed: 32915453
  • Tranilast abrogates cisplatin©\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation34047436

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