Polyclonal Antibody to Caspase 9 (CASP9) Rattus norvegicus (Rat) Polyclonal antibody

APAF3; ICE-LAP6; MCH6; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 9; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA627Ra01-Recombinant Caspase 9 (CASP9)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Caspase 9 (CASP9) Packages (Simulation)
  • Polyclonal Antibody to Caspase 9 (CASP9) Packages (Simulation)
  • Polyclonal Antibody to Caspase 9 (CASP9) DAB staining on IHC-P; Samples: Rat Stomach Tissue; Primary Ab: 10µg/ml Rabbit Anti-Rat CASP9 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 9 (CASP9) DAB staining on IHC-P;
    Samples: Rat Uterus Tissue;
    Primary Ab: 30ug/ml Rabbit Anti-Rat CASP9 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 9 (CASP9) DAB staining on IHC-P;
    Samples: Rat Kidney Tissue;
    Primary Ab: 30ug/ml Rabbit Anti-Rat CASP9 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 9 (CASP9) DAB staining on IHC-P;
    Samples: Rat Cardiac Muscle Tissue;
    Primary Ab: 30ug/ml Rabbit Anti-Rat CASP9 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA627Ra01.jpg Figure. Western Blot; Sample: Recombinant CASP9, Rat.
  • Polyclonal Antibody to Caspase 9 (CASP9) Western Blot; Sample: Lane1: Rat Uterus lysate; Lane2: Rat Placenta lysate; Lane3: Rat Bladder lysate; Lane4: Mouse Spleen lysate; Lane5: Mouse Testis lysate; Lane6: Porcine Uterus lysate; Lane7: 3T3-L1 cell lysate
    Primary Ab: 0.5μg/ml Rabbit Anti-Rat CASP9 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CASP9. It has been selected for its ability to recognize CASP9 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Role of mitochondria in ciprofloxacin-induced apoptosis in murine sperm cells.Pubmed: 23420823
  • Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial PerturbationsPubmed:25502462
  • Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells.pubmed:27461108
  • Stretch-induced apoptosis in rat alveolar epithelial cells is mediated by the intrinsic mitochondrial pathway.pubmed:28394655
  • Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cellspubmed:28231747
  • Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus.pubmed:28553224
  • Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathwayPubmed:29266336
  • Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino micePubmed: 31087429
  • Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activityPubmed: 31216504
  • Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cellsPubmed: 33206337
  • Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of …Pubmed: 32915453
  • Insights Into Protective Mechanisms of Dandelion Leaf Extract Against Cisplatin-Induced Nephrotoxicity in Rats: Role of Inhibitory Effect on Inflammatory and Apoptotic …
  • Potential cardioprotective effect of octreotide via NOXs mitigation, mitochondrial biogenesis and MAPK/Erk1/2/STAT3/NF-kβ pathway attenuation in isoproterenol …Pubmed:35500641

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