Polyclonal Antibody to Chloride Intracellular Channel Protein 4 (CLIC4) Mus musculus (Mouse) Polyclonal antibody

H1; CLIC4L; huH1; p64H1; Intracellular chloride ion channel protein p64H1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPF304Mu01-Recombinant Chloride Intracellular Channel Protein 4 (CLIC4)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.34mg/ml
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  • Polyclonal Antibody to Chloride Intracellular Channel Protein 4 (CLIC4) Packages (Simulation)
  • Polyclonal Antibody to Chloride Intracellular Channel Protein 4 (CLIC4) Packages (Simulation)
  • Polyclonal Antibody to Chloride Intracellular Channel Protein 4 (CLIC4) DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse CLIC4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Chloride Intracellular Channel Protein 4 (CLIC4) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse CLIC4 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAF304Mu01.jpg Figure. Western Blot; Sample: Recombinant CLIC4, Mouse.
  • Polyclonal Antibody to Chloride Intracellular Channel Protein 4 (CLIC4) Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Human PANC1 cell lysate;
    Primary Ab: 1µg/mL Rabbit Anti-Mouse CLIC4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CLIC4. It has been selected for its ability to recognize CLIC4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;1:170-700
Immunohistochemistry: 5-20µg/mL;1:17-70
Immunocytochemistry: 5-20µg/mL;1:17-70
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • Novel biomarkers of bronchopulmonary dysplasia and bronchopulmonary dysplasia-associated pulmonary hypertensionPubmed: 32811975

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