Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) Rattus norvegicus (Rat) Polyclonal antibody

CHR-NA1; N-AChRA1; NAChRA1; ACHRA; ACHRD; CMS2A; FCCMS; SCCMS; Acetylcholine receptor subunit alpha; N-AChR-A1; Neuronal Acetylcholine Receptor Alpha 1,Muscle

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA981Ra01-Recombinant Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) Packages (Simulation)
  • Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) Packages (Simulation)
  • Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) DAB staining on IHC-P; Sample: Rat Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat CHRNa1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) DAB staining on IHC-P;
    Sample: Rat Skeletal muscle Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat CHRNa1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CHRNa1. It has been selected for its ability to recognize CHRNa1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Effects of Endurance and Resistance Training on Calcitonin Gene-Related Peptide and Acetylcholine Receptor at Slow and Fast Twitch Skeletal Muscles and Sciatic Nerve in Male Wistar RatsPubMed: PMC3382945
  • Effects of vortioxetine on biomarkers associated with glutamatergic activity in an SSRI insensitive model of depression in female ratspubmed:29269186
  • Sukkari dates seed improves type-2 diabetes mellitus-induced memory impairment by reducing blood glucose levels and enhancing brain cholinergic …Pubmed:35812141

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