Polyclonal Antibody to Claudin 5 (CLDN5)     
     
AWAL; BEC1; CPETRL1; TMVCF; Transmembrane Protein Deleted In Velocardiofacial Syndrome; Transmembrane protein deleted in VCFS
Overview
Properties
- Product No.PAF295Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
 If the antibody is used in flow cytometry, please check FCM antibodies.
 Research use only
- DownloadInstruction Manual
- CategorySignal transduction
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen CPF295Hu21-OVA Conjugated Claudin 5 (CLDN5)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 0.5mg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against CLDN5. It has been selected for its ability to recognize CLDN5 in immunohistochemical staining and western blotting.
Usage
            Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.        
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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                 Antibody Labeling Customized Service Antibody Labeling Customized Service
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                 Protein A/G Purification Column Protein A/G Purification Column
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                 Staining Solution for Cells and Tissue Staining Solution for Cells and Tissue
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                 Positive Control  for Antibody Positive Control  for Antibody
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                 Tissue/Sections Customized Service Tissue/Sections Customized Service
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                 Phosphorylated Antibody Customized Service Phosphorylated Antibody Customized Service
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                 Western Blot (WB) Experiment Service Western Blot (WB) Experiment Service
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                 Immunohistochemistry (IHC) Experiment Service Immunohistochemistry (IHC) Experiment Service
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                 Immunocytochemistry (ICC) Experiment Service Immunocytochemistry (ICC) Experiment Service
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                 Flow Cytometry (FCM) Experiment Service Flow Cytometry (FCM) Experiment Service
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                 Immunoprecipitation (IP) Experiment Service Immunoprecipitation (IP) Experiment Service
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                 Immunofluorescence (IF) Experiment Service Immunofluorescence (IF) Experiment Service
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                 Buffer Buffer
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                 DAB Chromogen Kit DAB Chromogen Kit
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                 SABC Kit SABC Kit
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                 Long-arm Biotin Labeling Kit Long-arm Biotin Labeling Kit
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                 Real Time PCR Experimental Service Real Time PCR Experimental Service
Citations
- The Impact of the Endothelial Tight Junction Protein, Claudin-5, on the Clinical Profiles of Patients With COPDPubmed:30088372
- Serum zonulin and claudin-5 levels in children with attention-deficit/hyperactivity disorderPubmed: 32757874
- Serum zonulin and claudin-5 levels in patients with schizophreniaPubmed: 32564127
- Regulatory effect of lithium on hippocampal blood‐brain barrier integrity in a rat model of depressive‐like behaviorPubmed: 32558151
- Zonulin and claudin‐5 levels in multisystem inflammatory syndrome and SARS‐CoV‐2 infection in childrenPubmed:35638118

 
         
    
     
                 
                