Polyclonal Antibody to Cluster Of Differentiation 4 (CD4)

CD4mut; p55; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 128.00 US$ 300.00 US$ 428.00 US$ 1,070.00 US$ 4,280.00
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Overview

Properties

Product No.PAB167Si01
Organism SpeciesRhesus monkey (Simian) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction CD & Adhesion molecule Immune molecule

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB167Si01-Recombinant Cluster Of Differentiation 4 (CD4)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Human Spleen Tissue; Primary Ab: 20µg/ml Rabbit Anti-Simian CD4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant CD4, Simian.
Figure. Western Blot; Sample: Lane1: Human HL-60 Cells; Lane2: Mouse Spleen Tissue.

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CD4. It has been selected for its ability to recognize CD4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)

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Citations

Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation09540105.2017.1306494
Adjuvant Postoperative CD40 Agonist and PD-1 Antagonist Combination Therapy in Syngeneic Tongue Cancer Mouse ModelPubmed: 32366416