Polyclonal Antibody to Cluster Of Differentiation 83 (CD83) Mus musculus (Mouse) Polyclonal antibody

BL11; HB15; Activated B Lymphocytes,Immunoglobulin; B-cell activation protein; Cell surface protein HB15

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB845Mu01-Recombinant Cluster Of Differentiation 83 (CD83)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Cluster Of Differentiation 83 (CD83) Packages (Simulation)
  • Polyclonal Antibody to Cluster Of Differentiation 83 (CD83) Packages (Simulation)
  • PAB845Mu01.jpg Western Blot; Sample: Mouse Cerebellum lysate Primary Ab: 0.1µg/ml Rabbit Anti-Mouse CD83 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CD83. It has been selected for its ability to recognize CD83 in immunohistochemical staining and western blotting.

Usage

Western blottin:g: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Murine CD83-positive T cells mediate suppressor functions in vitro and in vivoPubmed:25151500
  • The Polyunsaturated Fatty Acids Arachidonic Acid and Docosahexaenoic Acid Induce Mouse Dendritic Cells Maturation but Reduce T-Cell Responses In VitroPubMed: 26619195
  • NK cells are negatively regulated by sCD83 in experimental autoimmune uveitispubmed:29038541
  • A Kinetic Study of CD83 Reveals an Upregulation and Higher Production of sCD83 in Lymphocytes from Pregnant Mice.pubmed:28491062
  • Soluble CD83 Alleviates Experimental Autoimmune Uveitis by Inhibiting Filamentous Actin-Dependent Calcium Release in Dendritic CellsPubmed:30050530
  • Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasionPubmed: 32810792

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