Polyclonal Antibody to Clusterin (CLU) Sus scrofa; Porcine (Pig) Polyclonal antibody

CLU; TRPM2; KUB1; CLI; APOJ; SGP2; AAG4; APO-J; SP-40; Apolipoprotein J; Complement Lysis Inhibitor; 40,Sulfated Glycoprotein 2; Testosterone-Repressed Prostate Message 2

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB180Po01-Recombinant Clusterin (CLU)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Clusterin (CLU) Packages (Simulation)
  • Polyclonal Antibody to Clusterin (CLU) Packages (Simulation)
  • Polyclonal Antibody to Clusterin (CLU) DAB staining on IHC-P; Sample: Porcine Pancreas Tissue; Primary Ab: 20ug/ml Rabbit Anti-Porcine CLU Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Clusterin (CLU) DAB staining on IHC-P;
    Sample: Porcine Liver Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Porcine CLU Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Clusterin (CLU) DAB staining on IHC-P;
    Sample: Porcine Uterus Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Porcine CLU Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Clusterin (CLU) DAB staining on IHC-P;
    Sample: Porcine Pancreas Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Porcine CLU Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Clusterin (CLU) DAB staining on IHC-P;
    Sample: Porcine Uterus Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Porcine CLU Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Clusterin (CLU) DAB staining on IHC-P;
    Sample: Porcine Liver Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Porcine CLU Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Clusterin (CLU) FITC staining on IF;
    Sample: Hela cell
    Primary Ab: 30µg/ml Rabbit Anti-Porcine CLU Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAB180Po01.jpg Figure. Western Blot; Sample: Recombinant CLU, Porcine.
  • Polyclonal Antibody to Clusterin (CLU) Western Blot; Sample: Porcine Serum;
    Primary Ab: 1µg/ml Rabbit Anti-Porcine CLU Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Clusterin (CLU) Western Blot; Sample: Human WERI-Rb-1 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Porcine CLU Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CLU. It has been selected for its ability to recognize CLU in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • Deficiency of Clusterin Exacerbates High-Fat Diet-Induced Insulin Resistance in Male Mice Pubmed: 24684302
  • Time-Dependent Decrease of Clusterin as a Potential Cerebrospinal Fluid Biomarker for Drug-Resistant Epilepsy.Pubmed: 24488574
  • Serum Amyloid A and Clusterin as Potential Predictive Biomarkers for Severe Hand, Foot and Mouth Disease by 2D-DIGE Proteomics AnalysisPubmed:25268271
  • Proteomic analysis of human plasma and peripheral blood mononuclear cells in Systemic Lupus Erythematosus patients.pubmed:28390925
  • Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ LabelingTechnology.pubmed:27911324
  • Regulatory effects of simvastatin and apoJ on APP processing and amyloid-β clearance in blood-brain barrier endothelial cellspubmed:28941799
  • Clusterin, a New Cerebrospinal Fluid Biomarker in Severe Subarachnoid Hemorrhage: A Pilot Studypubmed:28803177
  • Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysisPubmed:29386009
  • High serum clusterin levels are correlated with premature coronary artery disease in a Chinese populationPubmed: 30659732
  • Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer33602229

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