Monoclonal Antibody to Coagulation Factor II (F2)

FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 109.00 US$ 253.00 US$ 362.00 US$ 905.00 US$ 3,620.00
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Overview

Properties

Product No.MAA820Ra22
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction Hematology

SourceMonoclonal antibody preparation, Host Mouse
Ig Isotype IgG2b Kappa, Clone Number C13
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPA820Ra02-Recombinant Coagulation Factor II (F2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 1mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Rat Liver Tissue; Primary Ab: 20µg/ml Mouse Anti-Rat F2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Liver Tissue
Primary Ab: 20µg/ml Mouse Anti-Rat F2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
IHC Image

Figure. Western Blot; Sample: Recombinant F2, Rat.
Western Blot; Sample: Lane1: Rat Serum; Lane2: Rat Liver lysate; Lane3: Rat Spleen lysate
Primary Ab: 1µg/ml Mouse Anti-Rat F2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
WB Image
WB Image

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against F2. It has been selected for its ability to recognize F2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.