Monoclonal Antibody to Cofilin 1 (CFL1) Homo sapiens (Human) Monoclonal antibody

CFL; p18; N-Cofilin; 18 kDa phosphoprotein; Cofilin 1, Non Muscle

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2a Kappa, Clone Number C12
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB559Hu01-Recombinant Cofilin 1 (CFL1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Cofilin 1 (CFL1) Packages (Simulation)
  • Monoclonal Antibody to Cofilin 1 (CFL1) Packages (Simulation)
  • Monoclonal Antibody to Cofilin 1 (CFL1) DAB staining on IHC-P; Sample: Human Small intestine Tissue; Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cofilin 1 (CFL1) DAB staining on IHC-P;
    Sample: Human Liver Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cofilin 1 (CFL1) DAB staining on IHC-P;
    Sample: Human Spleen Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cofilin 1 (CFL1) DAB staining on IHC-P;
    Sample: Human Small intestine Tissue
    Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cofilin 1 (CFL1) DAB staining on IHC-P;
    Sample: Human Lung Tissue
    Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cofilin 1 (CFL1) DAB staining on IHC-P;
    Sample: Human Spleen Tissue
    Primary Ab: 20µg/ml Mouse Anti-Human CFL1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cofilin 1 (CFL1) AF488 staining on IF;
    Sample: Human Hela cell;
    Primary Ab: 30µg/ml Mouse Anti-Human CFL1 Antibody
    Second Ab: 2µg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • Monoclonal Antibody to Cofilin 1 (CFL1) FITC staining on IF;
    Sample: Hela cell
    Primary Ab: 30µg/ml Mouse Anti-Human CFL1 Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • MAB559Hu22.jpg Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Porcine Liver lysate Primary Ab: 2μg/ml Mouse Anti-Human CFL1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cofilin 1 (CFL1) Western Blot; Sample: Lane1: Bovine Cerebrum lysate; Lane2: HepG2 cell lysate
    Primary Ab: 0.2μg/ml Mouse Anti-Human CFL1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CFL1. It has been selected for its ability to recognize CFL1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle)

Giveaways

Citations

  • Detection of sputum cofilin-1 as indicator of malignancyPubmed:29846436
  • Hydroxytyrosol as a Promising Ally in the Treatment of FibromyalgiaPubmed: 32784915
  • The level of cytoskeleton remodeling proteins in the blood serum and the expression of their mRNA in the tumor tissue in metastasis of the larynx and hypopharynx cancer

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