Polyclonal Antibody to Collagen Type X (COL10)

COL10A1; COL10-A1; Schmid Metaphyseal Chondrodysplasia; Collagen Alpha-1(X)chain

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
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Overview

Properties

Product No.PAC156Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryMetabolic pathway Developmental science Nutrition metabolism Bone metabolism

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC156Hu01-Recombinant Collagen Type X (COL10)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 0.39mg/ml

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Packages (Simulation)
Packages (Simulation)

IHC Images
IHC Image
IHC Image

Western Blot; Sample: MCF7 cell lysate Primary Ab: 0.4µg/ml Rabbit Anti-Human COL10 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Figure. Western Blot; Sample: Human Cartilage Tissue.

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against COL10. It has been selected for its ability to recognize COL10 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Increment services

Citations

Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor IPubMed: 26123891
rAAV-mediated overexpression of sox9, TGF-β and IGF-I in minipig bone marrow aspirates to enhance the chondrogenic processes for cartilage repairPubMed: 26583804
Reduced Terminal Complement Complex Formation in Mice Manifests in Low Bone Mass and Impaired Fracture HealingPubmed: 30339839
Type X Collagen Assays and Methods of Use Thereof