Polyclonal Antibody to Complement Component 3 (C3) Homo sapiens (Human) Polyclonal antibody

ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA861Hu01-Recombinant Complement Component 3 (C3)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Complement Component 3 (C3) Packages (Simulation)
  • Polyclonal Antibody to Complement Component 3 (C3) Packages (Simulation)
  • Polyclonal Antibody to Complement Component 3 (C3) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human C3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 3 (C3) DAB staining on IHC-P;
    Samples: Human Mammary gland Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human C3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 3 (C3) Figure. DAB staining on IHC-P;
    Samples: Human Stomach Tissue.
  • Polyclonal Antibody to Complement Component 3 (C3) Figure. DAB staining on IHC-P;
    Samples: Human Thyroid Cancer Tissue.
  • Polyclonal Antibody to Complement Component 3 (C3) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human C3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA861Hu01.jpg Figure. Western Blot; Sample: Recombinant C3, Human.
  • Polyclonal Antibody to Complement Component 3 (C3) Western Blot; Sample: Lane1: Human Serum; Lane2: Human Plasma
    Primary Ab: 0.1μg/ml Rabbit Anti-Human C3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 3 (C3) Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human C3 Polyclonal Antibody (Catalog PAA861Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against C3. It has been selected for its ability to recognize C3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL
Immunohistochemistry: 5-30µg/mL
Immunocytochemistry: 5-30µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Experimental Research With Synthetic Copolymer-Coated Cardiopulmonary Bypass Circuits: Inflammatory and Thrombogenicity AnalysisWiley: source
  • Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease.PubMed: 22941511
  • Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteomePubMed: PMC3480889
  • Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo StudyPubMed: 26388133
  • Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculinPubmed:27032499
  • Identification of Serum Peptidome Signatures of Non-Small Cell Lung CancerPubmed:27043541
  • Comparative proteomics of milk fat globule membrane in goat colostrum and mature milkPubmed:27173528
  • Generation of complement protein C3 deficient pigs by CRISPR/Cas9-mediated gene targeting. pubmed:28694465
  • The response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain.pubmed:28888638
  • iTRAQ‐Based Proteomic Analysis Reveals Protein Profile in Plasma from Children with AutismPubmed:29274201
  • An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic Escherichia coli via Both Humoral and Predominantly Th1 Immune Responses in …Pubmed: 33233490
  • Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2JPubmed: 32585848
  • The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocytePubmed: 32555221
  • Complement component 3 prevents imiquimod-induced psoriatic skin inflammation by inhibiting apoptosis in micePubmed: 32535539
  • Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the …
  • A deep-sea pathogenic Bacillus subtilis isolate employs different strategies to escape the killing of teleost and murine complements33545212
  • Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves33610512
  • The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus
  • Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes34940989

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