Polyclonal Antibody to Corticosteroid Binding Globulin (CBG)
SERPINA6; Transcortin; Serpin A6; Serpin Peptidase Inhibitor,Clade A,Member 6(Alpha-1 Antiproteinase; Antitrypsin)
Overview
Properties
- Product No.PAB226Ra04
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEndocrinology
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB226Ra04-Recombinant Corticosteroid Binding Globulin (CBG)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against CBG. It has been selected for its ability to recognize CBG in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- The Regulation of Rasd1 Expression by Glucocorticoids and Prolactin Controls Peripartum Maternal Insulin SecretionEndjournals: Source
- Modulation in Wistar Rats of Blood Corticosterone Compartmentation by Sex and a Cafeteria DietPubMed: PMC3579843
- Differences in colocalization of corticosteroid-binding globulin and glucocorticoid receptor immunoreactivity in the rat brainScienceDirect: S0940960212001628
- Adrenocortical control in the neonatal rat: ACTH‐and cAMP‐independent corticosterone production during hypoxiaPubmed: 24303136
- Adrenocorticotropic Hormone But Not High-Density Lipoprotein Cholesterol or Salivary Cortisol Was a Predictor of Adrenal Insufficiency in Patients With Septic Shock.Pubmed: 24667620
- Greater glucocorticoid receptor activation in hippocampus of aged rats sensitizes microgliaPubmed:25559333
- Novel p53 target genes secreted by the liver are involved in non-cell-autonomous reCavia (Guinea pig )lationPubmed:26358154
- Impact of Season and Oral Contraceptive use on Cortisol Levelsin Physically Active Womenexercise-sports-orthopedics:exercise-sports-orthop
- Impact of Season and Oral Contraceptive use on Cortisol Levels in Physically Active Womendiva2:1047030
- Impaired glucocorticoid-mediated HPA axis negative feedback induced by juvenile social isolation in male ratsPubmed:29407214
- The impact of early-life stress on corticosteroid carrier protein levels and 11β-hydroxysteroid dehydrogenase 1 expression in adolescent rats