Polyclonal Antibody to Defensin Beta 1 (DEFb1) Rattus norvegicus (Rat) Polyclonal antibody

B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB373Ra01-Recombinant Defensin Beta 1 (DEFb1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Defensin Beta 1 (DEFb1) Packages (Simulation)
  • Polyclonal Antibody to Defensin Beta 1 (DEFb1) Packages (Simulation)
  • Polyclonal Antibody to Defensin Beta 1 (DEFb1) DAB staining on IHC-P; Samples: Rat Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat DEFb1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Defensin Beta 1 (DEFb1) DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat DEFb1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Defensin Beta 1 (DEFb1) DAB staining on IHC-P;
    Samples: Rat Liver cancer Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat DEFb1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB373Ra01.jpg Figure. Western Blot; Sample: Recombinant DEFb1, Rat.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against DEFb1. It has been selected for its ability to recognize DEFb1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Citations

  • Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell LineAsm: Source
  • Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han ChinesePubmed: 24124699
  • Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formulaDoi: 10.1111
  • Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells.Pubmed:26991948
  • Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host DefensePubmed:29997590
  • Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase …Pubmed: 30342347
  • EFFECT OF NIOSOMAL ANTIMICROBIAL PEPTIDE HBD-1 ON THE HEALING RATE OF INFECTED WOUNDS IN RATS
  • Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivoPubmed: 31313350
  • Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitisPubmed: 31927460
  • Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport33375218
  • Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus34362937
  • Isolation, culture, and identification of ceruminous gland cellsPubmed:35113280

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