Polyclonal Antibody to Neutrophil Elastase (NE) 
ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase
Overview
Properties
- Product No.PAA181Ca01
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseInfection immunity
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA181Ca01-Recombinant Neutrophil Elastase (NE)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 0.5mg/mL
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Canine Spleen Tissue; Primary Ab: 10µg/ml Rabbit Anti-Canine NE Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Canine Small intestine Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Canine NE Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Canine Spleen Tissue
Primary Ab: 10µg/ml Rabbit Anti-Canine NE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Canine Small intestine Tissue
Primary Ab: 10µg/ml Rabbit Anti-Canine NE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Canine Lung Tissue
Primary Ab: 10µg/ml Rabbit Anti-Canine NE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: Canine Bone marrow lysate; Lane2: Human Leukocyte lysate Primary Ab: 0.04µg/ml Rabbit Anti-Canine NE Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against NE. It has been selected for its ability to recognize NE in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Homo sapiens (Human)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal CirculationHindawi: 612848
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- The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in ratspubmed:27430552
- Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model.pubmed:28070608
- Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide.pubmed:28251688
- Targeted regulation of sympathetic activity in paraventricular nucleus reduces inducible ventricular arrhythmias in rats after myocardial infarction10.1016:j.jjcc.2018.06.003
- Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure RatsPubmed:29684507
- Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytesPubmed:28948423
- The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic …Pubmed:29379286
- Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring ratsPubmed: 30423388
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- Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively …Pubmed: 31474682
- MiR-608 Exerts Anti-inflammatory Effects by Targeting ELANE in MonocytesPubmed: 31749032
- Neutrophil Extracellular Traps Associate with Clinical Stages in Breast CancerPubmed: 31656990
- Inhibition of microRNA-146a attenuated heart failure in myocardial infarction ratsPubmed: 31763669
- Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver TransplantationPubmed: 31871555
- Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitisPubmed: 31927460
- Neurotransmitter system aberrations in patients with drug addictionPubmed: 32804296
- Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitisPubmed: 32376471
- Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed ratsPubmed: 32349626
- Macrophage Extracellular Traps Aggravate Iron Overload©\Related Liver Ischemia/Reperfusion Injury33959955
- Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling?¡33505502
- Depression of Non-Neuronal Cholinergic System May Play a Role in Co-Occurrence of Subjective Daytime Sleepiness and Hypertension in Patients with …34934375
- Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel SyndromePubmed:35088760
- A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation