Monoclonal Antibody to Endothelin 1 (EDN1) Homo sapiens (Human) Monoclonal antibody

ET1; PPET1; Preproendothelin-1; Big endothelin-1

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number C10
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA482Hu01-Recombinant Endothelin 1 (EDN1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Endothelin 1 (EDN1) Packages (Simulation)
  • Monoclonal Antibody to Endothelin 1 (EDN1) Packages (Simulation)
  • Monoclonal Antibody to Endothelin 1 (EDN1) Vector Red staining on IHC-P; Samples: Human Placenta Tissue; Primary Ab: 20µg/ml Mouse Anti-Human EDN1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Monoclonal Antibody
  • Monoclonal Antibody to Endothelin 1 (EDN1) DAB staining on IHC-P;
    Samples: Human Pancreatic cancer Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Human EDN1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Endothelin 1 (EDN1) DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Human EDN1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Endothelin 1 (EDN1) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Human EDN1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Endothelin 1 (EDN1) DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Human EDN1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAA482Hu22.jpg Figure. Western Blot; Sample: Recombinant EDN1, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against EDN1. It has been selected for its ability to recognize EDN1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sicknessAPS: source
  • CD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
  • Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1PubMed: PMC3695508
  • Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic micePubmed: 24212843
  • Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat modelPubmed:25476829
  • One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneysPubmed:24977654
  • The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disordersPubmed:25245337
  • Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal InstillationPubmed:25580880
  • Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioningSciencedirect:S0022480414004909
  • The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafilPubMed: 26023989
  • The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patientsPubMed: 26690031
  • Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal InstillationPubMed: 25580880
  • An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injuryPubMed: 26078283
  • Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndromescience:article
  • Cardiovascular Risk Factors and Markers of Atherosclerosis in Stable Kidney TransplantRecipients.pubmed:27496444
  • Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X.pubmed:27884380
  • Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactosepdfcopy:42552
  • Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose587e5b6508aed3826af45e2d.pdf
  • Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edemapubmed:27760029
  • Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndromearticle:S1872-9312(16)30085-0
  • Biochemical and Molecular Markers of Congenital and Senile Cataractous LensesDOI: 10.4172/2155-9929.1000318
  • A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats.AN=123819331
  • Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation10.1016/j.jff.2017.05.033
  • Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute …Pubmed:29809108
  • Isolated kidney perfusion: the influence of pulsatile flowPubmed:29301417
  • Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension …Pubmed: 30359461
  • Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
  • Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight …Pubmed: 30570214
  • Whitening effects of cosmetic formulation in the vascular component of skin pigmentationPubmed: 31074159
  • APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan
  • Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease
  • Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in ratsPubmed: 32616735
  • Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive RatsPubmed: 33133215
  • Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency34119534
  • Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti …34736077
  • Bai-Hu-Tang regulates endothelin-1 and its signalling pathway in vascular endothelial cells34752899
  • Post-stroke treatment of storax improves long-term outcomes of stroke in rats34343648
  • Laser Doppler flowmetry in assessing the effect of liraglutide on microcirculation in rats with absolute insulin deficiency

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