Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) Homo sapiens (Human) Polyclonal antibody

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) Packages (Simulation)
  • Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) Packages (Simulation)
  • Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human ECE1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) DAB staining on IHC-P;
    Samples: Human Prostate cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human ECE1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) DAB staining on IHC-P;
    Samples: Human Colorectal cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human ECE1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human ECE1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA483Hu01.jpg WB Image
  • Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) Western Blot; Sample: Porcine Lung lysate
    Primary Ab: 0.5μg/ml Rabbit Anti-Human ECE1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ECE1. It has been selected for its ability to recognize ECE1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Carbon monoxide attenuates bacteria-induced Endothelin-1 expression in second trimester placental explantsPubmed: 24731730
  • The Role of the Endothelin-1 Pathway as a Biomarker for Donor Lung Assessment in Clinical Ex Vivo Lung PerfusionSciencedirect:S1053249815000054
  • Endothelin-converting enzymes degrade α-synuclein and are reduced in dementia with Lewy bodies.pubmed:28171705

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