Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) Homo sapiens (Human) Monoclonal antibody

ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number C4
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA757Hu02-Recombinant Epidermal Growth Factor Receptor (EGFR)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) Packages (Simulation)
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) Packages (Simulation)
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 5ug/ml Mouse Anti-Human EGFR Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) DAB staining on IHC-P;
    Sample: Human Placenta Tissue;
    Primary Ab: 5ug/ml Mouse Anti-Human EGFR Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) DAB staining on IHC-P;
    Sample: Human Lung cancer Tissue;
    Primary Ab: 5ug/ml Mouse Anti-Human EGFR Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) DAB staining on IHC-P;
    Sample: Human Stomach Tissue;
    Primary Ab: 5ug/ml Mouse Anti-Human EGFR Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) DAB staining on IHC-P;
    Sample: Human Adrenal gland Tissue;
    Primary Ab: 5ug/ml Mouse Anti-Human EGFR Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) DAB staining on IHC-P;
    Sample: Human Liver cancer Tissue;
    Primary Ab: 5ug/ml Mouse Anti-Human EGFR Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) IF Image
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) IF Image
  • MAA757Hu22.jpg Western Blot; Sample: Lane1: Hela cell lysate; Lane2: MCF7 cell lysate; Lane3: A431 cell lysate; Lane4: Human Placenta lysate Primary Ab: 0.04µg/ml Mouse Anti-Human EGFR Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) WB Image
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) WB Image
  • Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR) WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against EGFR. It has been selected for its ability to recognize EGFR in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Nimotuzumab combined with gemcitabine and cisplatin as second-line chemotherapy for advanced non-small-cell lung cancerWiley: source
  • Increased Epidermal Growth Factor Receptor (EGFR) Associated with Hepatocyte Growth Factor (HGF) and Symptom Severity in Children with Autism …Pubmed:25249767
  • GC1118, an anti-EGFR antibody with a distinct binding epitope and superior inhibitory activity against high-affinity EGFR ligandsPubMed: 26586721
  • LINC01225 promotes occurrence and metastasis of hepatocellular carcinoma in an epidermal growth factor receptor-dependent pathwayPubmed:26938303
  • Microfluidic immunosensor based on mesoporous silica platform and CMK-3/poly-acrylamide-co-methacrylate of dihydrolipoic acid modified gold electrode for cancer biomarker detection.pubmed:28335979
  • EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatformDoi: 10.1016/j.talanta.2018.10.016
  • Design, synthesis, molecular docking and biological activity evaluation of some novel indole derivatives as potent anticancer active agents and apoptosis inducersPubmed: 30665034
  • Novel thienopyrimidine derivatives as dual EGFR and VEGFR-2 inhibitors: design, synthesis, anticancer activity and effect on cell cycle profilePubmed: 30919701
  • Design and synthesis of new benzoxazole/benzothiazole-phthalimide hybrids as antitumor-apoptotic agents
  • Synthesis and anticancer activity of bis-benzo [d][1, 3] dioxol-5-yl thiourea derivatives with molecular docking studyPubmed: 31288134
  • Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters …Pubmed: 31737175
  • Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicinePubmed: 33212364
  • Association between Serum Epidermal Growth Factor Receptor and Cyclooxygenase-2 Levels in Patients with Non-small Cell Carcinoma of Lung
  • Synthesis, EGFR-TK inhibition and anticancer activity of new quinoxaline derivatives
  • Humoral immune response to epidermal growth factor receptor in lung cancer33495907
  • Pyrazolo[3,4-d]pyrimidine-based dual EGFR T790M/HER2 inhibitors: Design, synthesis, structure¨Cactivity relationship and biological activity as potential antitumor and anticonvulsant agents33545637
  • Design, synthesis, antiproliferative evaluation, and molecular docking study of new quinoxaline derivatives as apoptotic inducers and EGFR inhibitors
  • Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions34914377
  • Discovery of new pyrimido [5, 4-c] quinolines as potential antiproliferative agents with multitarget actions: Rapid synthesis, docking, and ADME studiesPubmed:35219045

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