Polyclonal Antibody to Estrogen Receptor Alpha (ERa) Mus musculus (Mouse) Polyclonal antibody

ER-A1; ER; ESR; ESR1; ESRA; NR3A1; NR3-A1; Estrogen Receptor 1; Nuclear Receptor Subfamily 3,Group A,Member 1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB050Mu01-Recombinant Estrogen Receptor Alpha (ERa)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Estrogen Receptor Alpha (ERa) Packages (Simulation)
  • Polyclonal Antibody to Estrogen Receptor Alpha (ERa) Packages (Simulation)
  • Polyclonal Antibody to Estrogen Receptor Alpha (ERa) DAB staining on IHC-P; Samples: Mouse Uterus Tissue; Primary Ab: 20µg/ml Rabbit Anti-Mouse ERa Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Estrogen Receptor Alpha (ERa) DAB staining on IHC-P;
    Samples: Mouse Oviduct Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse ERa Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Estrogen Receptor Alpha (ERa) DAB staining on IHC-P;
    Samples: Mouse Small intestine Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse ERa Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB050Mu01.jpg Figure. Western Blot; Sample: Recombinant ERa, Mouse.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ERa. It has been selected for its ability to recognize ERa in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signallingPubmed:24846829
  • Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomasPubMed: 26529545
  • Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblastsscience:S1001074216300924
  • Influenza A virus NS1 protein-induced JNK activation and apoptosis are not functionally linked.pubmed:28076660
  • Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signalingpubmed:28263910
  • Antiestrogenic Activity of the Xi-Huang Formula for Breast Cancer by Targeting the Estrogen Receptor αPubmed:29975948
  • In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts
  • Vitamin D receptor in breast cancer tissues and its relation to estrogen receptor alpha (ER-a) gene expression and serum 25-hydroxyvitamin D levels in Egyptian Doi: 10.1016/j.clbc.2018.12.019
  • ERα Gene Promoter Methylation in Cognitive Function and Quality of Life of Patients With Alzheimer DiseasePubmed: 30947592
  • The Effector Domain of the Influenza A Virus Nonstructural Protein NS1 Triggers Host Shutoff by Mediating Inhibition and Global Deregulation of Host Transcription …34488451
  • Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue

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