Polyclonal Antibody to Fascin (FSCN) Rattus norvegicus (Rat) Polyclonal antibody

FSCN1; SNL; p55; Singed-like protein; 55 kDa actin-bundling protein; Fascin homolog 1, Actin-Bundling Protein(Strongylocentrotus Purpuratus); Singed,Drosophila,Homolog-Like

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB757Ra01-Recombinant Fascin (FSCN)
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Fascin (FSCN) Packages (Simulation)
  • Polyclonal Antibody to Fascin (FSCN) Packages (Simulation)
  • Polyclonal Antibody to Fascin (FSCN) DAB staining on IHC-P; Sample: Rat Testis Tissue Primary Ab: 20µg/ml Rabbit Anti-Rat FSCN Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Fascin (FSCN) DAB staining on IHC-P;
    Sample: Rat Spleen Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Rat FSCN Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB757Ra01.jpg Figure. Western Blot; Sample: Recombinant FSCN, Rat.
  • Polyclonal Antibody to Fascin (FSCN) Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Rat FSCN Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Fascin (FSCN) Western Blot; Sample: Rat Lung lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Rat FSCN Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against FSCN. It has been selected for its ability to recognize FSCN in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)

Giveaways

Citations

  • Serological investigation of the clinical significance of fascin in non-small-cell lung cancerScienceDirect: S0169500213003826
  • Fascin-1 is released from proximal tubular cells in response to calcineurin inhibitors (CNIs) and correlates with isometric vacuolization in kidney transplanted patientsPMC5622260
  • Clinical significance of fascin-1 and laminin-5 in non-small cell lung cancer.pubmed:28653739
  • ЦИркУлИрУЮщИе актИН-СвЯЗЫваЮщИе БелкИ ПрИ ПроГреССИроваНИИ рака ГортаНИ И ГортаНоГлоткИ
  • Fascin-1 and its role as a serological marker in prostate cancer: a prospective case–control study34737886

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