Polyclonal Antibody to Fibrinogen Beta Chain (FGB)

FG-B; Fibrinopeptide B; Fibrinogen Beta

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 100.00 US$ 232.00 US$ 332.00 US$ 830.00 US$ 3,320.00
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Overview

Properties

Product No.PAB215Hu03
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction Infection immunity Hematology

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB215Hu03-Recombinant Fibrinogen Beta Chain (FGB)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

Used in DAB staining on fromalin fixed paraffin-embedded Liver tissue

Figure. Western Blot; Sample: Recombinant FGb, Human.
Figure. Western Blot; Sample: Human Liver Tissue.
Western Blot; Sample: Human Serum;
Primary Ab: 1µg/ml Rabbit Anti-Human FGb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human FGB Polyclonal Antibody (Catalog PAB215Hu03, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against FGB. It has been selected for its ability to recognize FGB in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

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Citations

Compositions and Methods for Diagnosing Lung Cancery2016:0077095.html
Crystal structure of GAPDH of Streptococcus agalactiae and characterization of its interaction with extracellular matrix moleculesPubmed: 30553015
Nattokinase-heparin exhibits beneficial efficacy and safety—an optimal strategy for CKD patients on hemodialysisPubmed: 30788657