Polyclonal Antibody to Follistatin Like Protein 1 (FSTL1)

FRP; FSL1; Follistatin-related protein 1

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
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Overview

Properties

Product No.PAJ085Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryReproductive science

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPJ085Hu01-Recombinant Follistatin Like Protein 1 (FSTL1)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 0.38mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Human Liver Tissue Primary Ab: 10µg/ml Rabbit Anti-Human FSTL1 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human FSTL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human FSTL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human FSTL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human FSTL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: U2OS cell
Primary Ab: 20µg/ml Rabbit Anti-Human FSTL1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Western Blot; Sample: U2OS cell lysate Primary Ab: 0.3µg/ml Rabbit Anti-Human FSTL1 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human FSTL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Heart lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human FSTL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Placenta lysate
Primary Ab: 2µg/ml Rabbit Anti-Human FSTL1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against FSTL1. It has been selected for its ability to recognize FSTL1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;1:190-760;
Immunohistochemistry: 5-20µg/mL;1:19-76;
Immunocytochemistry: 5-20µg/mL;1:19-76;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

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Citations

Serum S100A8/A9, but not follistatin-like protein 1 and interleukin 18, may be a useful biomarker of disease activity in adult-onset Still's disease.PubMed: 22660800
Regulation of follistatin-like protein 1 expression and secretion in primary human skeletal muscle cellsPubmed: 23419164
Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferationPubmed:25118038
Growth Differentiation Factor-15 (GDF-15) Levels Are Associated with Cardiac and Renal Injury in Patients Undergoing Coronary Artery Bypass Grafting with Cardiopulmonary BypassPubmed:25171167
Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistancePubMed: 26199111
Association of Circulating Follistatin-Like 1 Levels with Inflammatory and Oxidative Stress Markers in Healthy Menpmc:PMC4856269
Follistatin like 1 Regulates Hypertrophy in Heart Failure with Preserved Ejection Fraction.pubmed:27430031
Follistatin like-1 aggravates silica-induced mouse lung injuryPMC5428474
Plasma follistatin-like protein 1 is correlated with disease severity in patients with acute pulmonary embolism and predicts short-term mortalityISSN:1936-2625
High circulating follistatin-like protein 1 as a biomarker of a metabolically unhealthy statePubmed: 30745500
Associations among circulating levels of follistatin-like 1, clinical parameters, and cardiovascular events in patients undergoing elective percutaneous …Pubmed: 31034503
Improved early detection of ovarian cancer using longitudinal multimarker modelsPubmed: 31937926
Targeting Follistatin like 1 ameliorates liver fibrosis induced by carbon tetrachloride through TGF-β1-miR29a in micePubmed: 32933544
Effects of acute endurance exercise on follistatin-like 1 and apelin in the circulation and metabolic organs in ratsPubmed: 32412800
Prognostic impact of transcardiac gradient of follistatin-like 1 reflecting hemodynamics in patients with dilated cardiomyopathy34334268
Association between Plasma Follistatin-like Protein 1 Levels and the Presence and Severity of Coronary Artery Disease34853217