Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) 
GSHPX3; GPx-P; GSHPx-P; GPXP; Plasma glutathione peroxidase; Extracellular glutathione peroxidase
Overview
Properties
- Product No.PAC992Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & Kinase
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPC992Hu01-Recombinant Glutathione Peroxidase 3, Plasma (GPX3)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 0.5mg/mL
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Prostate cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human GPX3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human GPX3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human GPX3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human GPX3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human GPX3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Pancreatic cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human GPX3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant GPX3, Human.
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Western Blot; Sample: Human Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human GPX3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against GPX3. It has been selected for its ability to recognize GPX3 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Alteration in the intrafollicular thiol–redox system in infertile women with endometriosisPubmed:25376627
- Endothelial function impairment in STEMI patients with out-of-hospital cardiac arrest under therapeutic hypothermia treatment.pubmed:28087172
- Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients.pubmed:27748187
- Burn Serum Increases Staphylococcus aureus Biofilm Formation via Oxidative Stress.pubmed:28702016
- Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1Pubmed: 30284230
- Kidney toxicity and response of selenium containing protein-glutathione peroxidase (Gpx3) to CdTe QDs on different levelsPubmed: 30535317
- Human Plasma Protein (s) Based Conceptual Diagnostic Tool for Altitude-Acclimation Assessment
- Dietary selenium deficiency or selenomethionine excess drastically alters organ selenium contents without altering the expression of most selenoproteins in mice
- Molecular mechanisms of lead-induced changes of selenium status in mice livers through interacting with selenoprotein P
- In vivo and in vitro studies on inactivation of selenium containing protein-glutathione peroxidase 3 in mice nephrocytes caused by leadPubmed: 32678766
- Selenium–GPX4 axis protects follicular helper T cells from ferroptosis34413521
- Experimental nephrotic syndrome in mice with an inducible deletion of podocin and the role of plasminogen in sodium retention