Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) Homo sapiens (Human) Polyclonal antibody

GPLL; GPBB; Hers Disease; Glycogen Storage Disease Type VI

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA849Hu01-Recombinant Glycogen Phosphorylase, Liver (PYGL)
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) Packages (Simulation)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) Packages (Simulation)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) DAB staining on IHC-P;
    Samples: Human Thyroid Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) DAB staining on IHC-P;
    Samples: Human Rectum Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) DAB staining on IHC-P;
    Samples: Human Lung Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) DAB staining on IHC-P;
    Sample: Human Placenta Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) DAB staining on IHC-P;
    Sample: Porcine Cerebrum Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) DAB staining on IHC-P;
    Sample: Porcine Liver Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) FITC staining on IF;
    Samples: Human HepG2 cell;
    Primary Ab: 20μg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) FITC staining on IF;
    Sample: HepG2 cell
    Primary Ab: 20µg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA849Hu01.jpg Figure. Western Blot; Sample: Recombinant PYGL, Human.
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) Western Blot; Sample: Human Liver lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) Western Blot; Sample: Mouse Cerebrum lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) Western Blot; Sample: Human BXPC3 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Liver (PYGL) Western Blot; Samples: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: HepG2 cell lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Human PYGL Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PYGL. It has been selected for its ability to recognize PYGL in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

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