Polyclonal Antibody to Glycogen Phosphorylase, Muscle (PYGM) Mus musculus (Mouse) Polyclonal antibody

GPMM; Phosphorylase,Glycogen; McArdle Syndrome,Glycogen Storage Disease Type V; Myophosphorylase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA848Mu01-Recombinant Glycogen Phosphorylase, Muscle (PYGM)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Glycogen Phosphorylase, Muscle (PYGM) Packages (Simulation)
  • Polyclonal Antibody to Glycogen Phosphorylase, Muscle (PYGM) Packages (Simulation)
  • Polyclonal Antibody to Glycogen Phosphorylase, Muscle (PYGM) DAB staining on IHC-P; Sample: Mouse Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Mouse PYGM Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked C
  • Polyclonal Antibody to Glycogen Phosphorylase, Muscle (PYGM) DAB staining on IHC-P;
    Sample: Mouse Skeletal muscle Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse PYGM Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
    Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Muscle (PYGM) FITC staining on IF;
    Samples: Human HepG2 cell;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse PYGM Antibody
    Second Ab: 1.5µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Glycogen Phosphorylase, Muscle (PYGM) FITC staining on IF;
    Sample: HepG2 cell
    Primary Ab: 20µg/ml Rabbit Anti-Mouse PYGM Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA848Mu01.jpg Figure. Western Blot; Sample: Recombinant PYGM, Mouse.
  • Polyclonal Antibody to Glycogen Phosphorylase, Muscle (PYGM) Western Blot; Sample: Mouse Skeletal muscle lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Mouse PYGM Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Muscle (PYGM) Western Blot; Sample: Rat Skeletal muscle lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Mouse PYGM Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Muscle (PYGM) Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Mouse PYGM Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Phosphorylase, Muscle (PYGM) Western Blot; Sample: Mouse Liver lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Mouse PYGM Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PYGM. It has been selected for its ability to recognize PYGM in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

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