Polyclonal Antibody to Glycogen Synthase Kinase 3 Beta (GSK3b) Rattus norvegicus (Rat) Polyclonal antibody

Serine/threonine-protein kinase GSK3B

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPD317Ra01-Recombinant Glycogen Synthase Kinase 3 Beta (GSK3b)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Glycogen Synthase Kinase 3 Beta (GSK3b) Packages (Simulation)
  • Polyclonal Antibody to Glycogen Synthase Kinase 3 Beta (GSK3b) Packages (Simulation)
  • Polyclonal Antibody to Glycogen Synthase Kinase 3 Beta (GSK3b) DAB staining on IHC-P;
    Sample: Rat Cerebellum Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat GSK3b Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Synthase Kinase 3 Beta (GSK3b) DAB staining on IHC-P;
    Sample: Rat Spinal cord Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat GSK3b Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glycogen Synthase Kinase 3 Beta (GSK3b) Western Blot; Sample: Rat Cerebrum lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Rat GSK3b Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against GSK3b. It has been selected for its ability to recognize GSK3b in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Sorafenib and lithium chloride combination treatment shows promising synergistic effects in human glioblastoma multiforme cells in vitro but midkine is not implicatedManeyonline:Source
  • Glycogen synthase kinase-3β (GSK-3β) rs334558 polymorphism is not associated with mild cognitive impairment in Chinese Han type 2 diabetic patientsijcem0038535.pdf
  • Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate HypoxiaPubmed:29308565
  • Neuroprotective effects of Bergenia ciliata on NMDA induced injury in SH-SY5Y cells and attenuation of cognitive deficits in scopolamine induced amnesia in ratsPubmed: 30227331
  • FEATURES OF DISTRIBUTION OF SOYBEAN AGGLUTININ (SBA) RECEPTORS IN THE EXTRACELLULAR MATRIX OF THE MENISCI OF RAT KNEE JOINT …
  • Glycogen synthase kinase 3β/CCR6‐positive bone marrow cells correlate with disease activity in multicentric Castleman disease‐TAFRO34873687

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