Polyclonal Antibody to Golgi Phosphoprotein 2 (GOLPH2) Mus musculus (Mouse) Polyclonal antibody

GOLM1; GP73; C9orf155; Golgi Membrane Protein 1; Golgi Protein 73

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB668Mu01-Recombinant Golgi Phosphoprotein 2 (GOLPH2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Polyclonal Antibody to Golgi Phosphoprotein 2 (GOLPH2) Packages (Simulation)
  • Polyclonal Antibody to Golgi Phosphoprotein 2 (GOLPH2) Packages (Simulation)
  • Polyclonal Antibody to Golgi Phosphoprotein 2 (GOLPH2) DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse GP73 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Golgi Phosphoprotein 2 (GOLPH2) DAB staining on IHC-P;
    Samples: Mouse Cardiac Muscle Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse GP73 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Golgi Phosphoprotein 2 (GOLPH2) DAB staining on IHC-P;
    Samples: Mouse Uterus Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse GP73 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Golgi Phosphoprotein 2 (GOLPH2) DAB staining on IHC-P;
    Samples: Mouse Small intestine Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse GP73 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Golgi Phosphoprotein 2 (GOLPH2) DAB staining on IHC-P;
    Samples: Mouse Testis Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse GP73 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Golgi Phosphoprotein 2 (GOLPH2) DAB staining on IHC-P;
    Samples: Mouse Colon Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse GP73 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB668Mu01.jpg Figure. Western Blot; Sample: Recombinant GP73, Mouse.
  • Polyclonal Antibody to Golgi Phosphoprotein 2 (GOLPH2) Western Blot; Sample: Lane1: Mouse Stomach lysate; Lane2: Mouse Trachea lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Mouse IGF1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Golgi Phosphoprotein 2 (GOLPH2) Mouse Hepa1-6 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-Mouse GOLPH2 Polyclonal Antibody (Catalog PAB668Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against GOLPH2. It has been selected for its ability to recognize GOLPH2 in immunohistochemical staining and western blotting.

Usage

Western blottin:g: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • LAMC2: A promising new pancreatic cancer biomarker identified by proteomic analysis of pancreatic adenocarcinoma tissuesMcponline: 023507
  • Diagnostic and Prognostic Validity of Serum Golgi Protein 73 in Egyptian Patients with Hepatocelluar CarcinomaAjied: Ajied_System_Files
  • Diagnostic role of Golgi protein 73 and IL-17 in Egyptian cirrhotic rather than hepatocellular carcinoma patients.handle:123456789
  • Serum Golgi protein 73 is a prognostic rather than diagnostic marker in hepatocellular carcinomapubmed:29113278
  • The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian studyPubmed: 32198440
  • Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformationPubmed: 32171652
  • Deoxycholic Acid Upregulates Serum Golgi Protein 73 through Activating NF-¦ÊB Pathway and Destroying Golgi Structure in Liver Disease. Biomolecules 2021, 11?¡­33540642
  • Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C

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