Polyclonal Antibody to Gremlin 1 (GREM1) Chicken (Gallus) Polyclonal antibody

PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC128Ga01-Recombinant Gremlin 1 (GREM1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Gremlin 1 (GREM1) Packages (Simulation)
  • Polyclonal Antibody to Gremlin 1 (GREM1) Packages (Simulation)
  • PAC128Ga01.jpg Figure. Western Blot; Sample: Recombinant GREM1, Chicken.
  • Polyclonal Antibody to Gremlin 1 (GREM1) Western Blot; Sample: Mouse Testis lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Gallus GREM1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against GREM1. It has been selected for its ability to recognize GREM1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

Citations

  • Intrinsic BMP Antagonist Gremlin-1 as a Novel Circulating Marker in Pulmonary Arterial HypertensionPubMed: 25926293
  • Overexpression of Gremlin promotes non-small cell lung cancer progressionPubMed: 26392110
  • Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-term prognosis in suspected and proven acute heart failure patientsPubMed: 26539964
  • Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-termprognosis in suspected and proven acute heart failure patientsPubmed:26539964
  • BMPR2 Mutation-independent Mechanisms of Disrupted BMP Signaling in IPAHPubmed:27187737
  • GREM1 Is a Key ReCavia (Guinea pig )lator of Synoviocyte Hyperplasia and InvasivenessPubmed:26834210
  • The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells.pubmed:28266688
  • Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients.pubmed:28327551
  • Bone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial …rcmb.2015-0402OC
  • Gremlin-1 suppression increases BMP-2-induced osteogenesis of human mesenchymal stem cellspubmed:28260028
  • Platelets as a novel source of Gremlin-1: Implications for thrombo inflammationpubmed:27929199
  • Gremlin, A Potential Urinary Biomarker of Anca-Associated Crescentic GlomerulonephritisPubmed: 31053735
  • Transcriptomic and Proteomic Analyses Reveal the Potential Mode of Action of Chondrocyte Sheets in Hyaline Cartilage RegenerationPubmed: 31878307
  • Suppression of microRNA-23a/b-3p delays the development degenerative joint disease by activating Grem1 and TGF-β/Smad signaling pathwayPubmed: 32186934
  • Protective role of microRNA-23a/b-3p inhibition against osteoarthritis through Gremlin1-depenent activation of TGF-β/smad signaling in chondrocytesaPubmed:35441352
  • CAF‐derived exosomes transmitted Gremlin‐1 promotes cancer progression and decreases the sensitivity of hepatoma cells to sorafenibPubmed:35638711

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