Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Bos taurus; Bovine (Cattle) Polyclonal antibody

HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB081Bo01-Recombinant Heat Shock 70kDa Protein 1A (HSPA1A)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) DAB staining on IHC-P; Samples: Bovine Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Bovine HSPA1A Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) DAB staining on IHC-P;
    Samples: Bovine Testis Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Bovine HSPA1A Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) DAB staining on IHC-P;
    Sample: Bovine Testis Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Bovine HSPA1A Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) DAB staining on IHC-P;
    Sample: Bovine Liver Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Bovine HSPA1A Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB081Bo01.jpg Figure. Western Blot; Sample: Recombinant HSPA1A, Bovine.
  • Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Western Blot; Sample: Bovine Cerebrum lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Bovine HSPA1A Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Western Blot; Sample: Bovine Kidney lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Bovine HSPA1A Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Western Blot; Sample: Bovine Liver lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Bovine HSPA1A Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Western Blot; Samples: Lane1: Bovine Cerebrum lysate; Lane2: Bovine Liver lysate; Lane3: Bovine Kidney lysate; Lane4: HepG2 cell lysate; Lane5: PC3 cell lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Bovine HSPA1A Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HSPA1A. It has been selected for its ability to recognize HSPA1A in immunohistochemical staining and western blotting.

Usage

Western blottin:g: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

Citations

  • Identification of potential biomarkers of gold nanoparticle toxicity in rat brainsPubMed: 22691312
  • Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in PigsPubmed:Pmc4324980
  • Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in ratsPubmed:24508522
  • Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing micePubmed:25443743
  • A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmaticspubmed:28822267
  • Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young …Pubmed:29379533
  • Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampusPubmed: 32172517
  • Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in micePubmed: 32439493

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