Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) Homo sapiens (Human) Polyclonal antibody

HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB831Hu01-Recombinant Hedgehog Homolog, Sonic (SHH)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) Packages (Simulation)
  • Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) Packages (Simulation)
  • Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human SHH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human SHH Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) DAB staining on IHC-P;
    Samples: Human Brain Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human SHH Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) AF488 staining on IF;
    Sample: HepG2 cell
    Primary Ab: 20µg/ml Rabbit Anti-Human SHH Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAB831Hu01.jpg Figure. Western Blot; Sample: Recombinant SHH, Human.
  • Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) Figure. Western Blot; Sample: Lane1: Human Liver Tissue; Lane2: Mouse Kidney Tissue; Lane3: Human PANC-1 Cells.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.

Usage

Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Citations

  • Compositions and Methods for Diagnosing Lung Cancery2016:0077095.html
  • Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosispubmed:28495672

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