Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) Homo sapiens (Human) Polyclonal antibody

PLC; PRCAN; SJA; SJS; SJS1; Perlecan; Endorepellin; Schwartz-Jampel Syndrome 1; Basement membrane-specific heparan sulfate proteoglycan core protein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC748Hu01-Recombinant Heparan Sulfate Proteoglycan 2 (HSPG2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) Packages (Simulation)
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) Packages (Simulation)
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human HSPG2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human HSPG2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) DAB staining on IHC-P;
    Samples: Porcine Spleen Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human HSPG2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) DAB staining on IHC-P;
    Samples: Porcine Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human HSPG2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) DAB staining on IHC-P;
    Samples: Porcine Cardiac Muscle Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human HSPG2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) DAB staining on IHC-P;
    Samples: Porcine Stomach Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human HSPG2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) FITC staining on IF;
    Sample: Human U2OS cell;
    Primary Ab: 30µg/ml Rabbit Anti-Human HSPG2 Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • PAC748Hu01.jpg Figure. Western Blot; Sample: Recombinant HSPG2, Human.
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) Western Blot; Sample: Porcine Heart lysate;
    Primary Ab: 5µg/ml Rabbit Anti-Human HSPG2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) Western Blot; Sample: Rat Heart lysate;
    Primary Ab: 5µg/ml Rabbit Anti-Human HSPG2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) Western Blot; Sample: Mouse Heart lysate;
    Primary Ab: 5µg/ml Rabbit Anti-Human HSPG2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heparan Sulfate Proteoglycan 2 (HSPG2) Western Blot; Sample: Mouse Lung lysate;
    Primary Ab: 5µg/ml Rabbit Anti-Human HSPG2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HSPG2. It has been selected for its ability to recognize HSPG2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-5µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzymePubmed: 24530413
  • Proteomics of Follicular Fluid From Women With Polycystic Ovary Syndrome Suggests Molecular Defects in Follicular DevelopmentPubmed:25393639
  • Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation.pubmed:27456745
  • Expression and Circulating Levels of Perlecan in Breast Cancer-Implications for Oestrogen Dependent Stromal RemodelingPubmed: 32124140

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