KO-Validated
Polyclonal Antibody to Heparanase (HPSE)

HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 95.00 US$ 221.00 US$ 316.00 US$ 790.00 US$ 3,160.00
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Overview

Properties

Product No.PAA711Hu02
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA711Hu02-Recombinant Heparanase (HPSE)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Placenta Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human HPA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Spleen Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human HPSE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human HPA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20µg/ml Rabbit Anti-Human HPA Antibody
Second Ab: 1.5µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Human HPSE Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant HPA, Human.
Western Blot; Samples: Lane1: LO2 cell lysate; Lane2: HepG2 cell lysate; Lane3: Jurkat cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human HPSE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: HPA knockout HepG2 cell lysate;
Predicted MW: 43,53,55,61kd
Observed MW: 68kd
Primary Ab: 1µg/ml Rabbit Anti-Human HPA Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HPSE. It has been selected for its ability to recognize HPSE in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Increment services

Citations

PG545, a Heparan Sulfate Mimetic, Reduces Heparanase Expression In Vivo, Blocks Spontaneous Metastases and Enhances Overall Survival in the 4T1 Breast Carcinoma ModelPlosone: Source
Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity ? uid in women with impaired reproductionNowinylekarskie:Source
Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanasePubMed: 26191183
PI-88 inhibits postoperative recurrence of hepatocellular carcinoma via disrupting the surge of heparanase after liver resectionPubMed: 26415733
Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity fluid in women with impaired reproductionarticle:84
Plasma heparanase is associated with blood glucose levels but not urinary microalbumin excretion in type 2 diabetic nephropathy at the early stage pubmed:28994624

KO-Validated Polyclonal Antibody to Heparanase (HPSE)