Polyclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)    
    
HB-EGF; DTR; DTS; DTSF; HEGFL; Diphtheria Toxin Receptor; Proheparin-binding EGF-like growth factor; Diphtheria toxin receptor
Overview
Properties
- Product No.PAB479Hu01
 - Organism SpeciesHomo sapiens (Human) Same name, Different species.
 - ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
 - CategoryCytokineTumor immunityInfection immunity
 
- SourcePolyclonal antibody preparation, Host Rabbit
 - Ig Type IgG, Potency n/a
 - PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
 - LabelNone
 - Immunogen RPB479Hu01-Recombinant Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)
 - Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
 - TraitsLiquid, Concentration 500µg/mL
 
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Specifity
The antibody is a rabbit polyclonal antibody raised against HBEGF. It has been selected for its ability to recognize HBEGF in immunohistochemical staining and western blotting.
Usage
            Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.        
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Sus scrofa; Porcine (Pig)Giveaways
Increment services
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                    Antibody Labeling Customized Service
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                    Protein A/G Purification Column
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                    Staining Solution for Cells and Tissue
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                    Positive Control  for Antibody
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                    Tissue/Sections Customized Service
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                    Phosphorylated Antibody Customized Service
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                    Western Blot (WB) Experiment Service
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                    Immunohistochemistry (IHC) Experiment Service
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                    Immunocytochemistry (ICC) Experiment Service
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                    Flow Cytometry (FCM) Experiment Service
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                    Immunoprecipitation (IP) Experiment Service
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                    Immunofluorescence (IF) Experiment Service
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                    Buffer
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                    DAB Chromogen Kit
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                    SABC Kit
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                    Long-arm Biotin Labeling Kit
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                    Real Time PCR Experimental Service
                                 
Citations
- Astrocyte ERK phosphorylation precedes K+-induced swelling but follows hypotonicity-induced swelling.PubMed: 21118399
 - Cypermethrin Induces Astrocyte Apoptosis by the Disruption of the Autocrine/Paracrine Mode of Epidermal Growth Factor Receptor SignalingPubmed: source
 - 軽度炎症性膀胱痛マウスモデルの構築とNGFの関与Jstage:Source
 - A novel method for assessing bladder-related pain reveals the involvement of nerve growth factor in painassociated with cyclophosphamide-induced chronic cystitis in mice.Pubmed:25820250
 - 2 型糖尿病モデルラットにおける糖尿病性腎症の進行とHB-EGF の関連性17844
 - Heparin-binding epidermal growth factor contributes to COPD disease severity by modulating airway fibrosis and pulmonary epithelial–mesenchymal transitionPubmed:29581578
 - CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17
 - Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growthPubmed: 30769285
 - Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheetsPubmed: 33164313
 - 象牙芽細胞の枯渇は象牙芽細胞分化と象牙質形成を誘導する
 
