Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)

HMG1L2; High-Mobility Group Protein 1-Like 2

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 97.00 US$ 227.00 US$ 324.00 US$ 810.00 US$ 3,240.00
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Overview

Properties

Product No.PAA624Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryCytokine Tumor immunity Infection immunity Hepatology

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA624Mu01-Recombinant Hepatoma Derived Growth Factor (HDGF)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 0.38mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Mouse Uterus Tissue; Primary Ab: 30µg/ml Rabbit Anti-Mouse HDGF Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse HDGF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Heart Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse HDGF Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Spleen Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse HDGF Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse HDGF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse HDGF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Mouse HDGF Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Figure. Western Blot; Sample: Recombinant HDGF, Mouse.
Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Mouse Testis lysate; Lane4: Jurkat cell lysate; Lane5: 293T cell lysate;
Primary Ab: 0.2µg/ml Rabbit Anti-Mouse HDGF Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
WB Image
WB Image
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse HDGF Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HDGF. It has been selected for its ability to recognize HDGF in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

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Citations

Hepatoma-derived growth factor promotes the resistance to anti-tumor effects of nordihydroguaiaretic acid in colorectal cancer cellsScienceDirect: S0014299910007302
Association of serum hepatoma‐derived growth factor levels with disease activity in rheumatoid arthritis: A pilot studyPubmed:35500218