Polyclonal Antibody to Hexosaminidase A Alpha (HEXa) Mus musculus (Mouse) Polyclonal antibody

TSD; Hex-A; Tay Sachs Disease; GM2 Gangliosidosis; Beta-Hexosaminidase Subunit Alpha; Beta-N-Acetylhexosaminidase Subunit Alpha; N-Acetyl-Beta-Glucosaminidase Alpha

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA195Mu01-Recombinant Hexosaminidase A Alpha (HEXa)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Hexosaminidase A Alpha (HEXa) Packages (Simulation)
  • Polyclonal Antibody to Hexosaminidase A Alpha (HEXa) Packages (Simulation)
  • Polyclonal Antibody to Hexosaminidase A Alpha (HEXa) Used in DAB staining on fromalin fixed paraffin-embedded Liver tissue
  • PAA195Mu01.jpg Figure. Western Blot; Sample: Recombinant HEXa, Mouse.
  • Polyclonal Antibody to Hexosaminidase A Alpha (HEXa) Western Blot; Sample: Mouse Testis lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Mouse HEXa Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HEXa. It has been selected for its ability to recognize HEXa in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Functionality of a bicistronic construction containing HEXA and HEXB genes encoding ¦Â-hexosaminidase A for cell-mediated therapy of GM2 gangliosidoses34100447
  • Serum Cytokine Profile, Beta-Hexosaminidase A Enzymatic Activity and GM2 Ganglioside Levels in the Plasma of a Tay-Sachs Disease Patient after Cord Blood Cell …34685379

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