• KO-Validated
Polyclonal Antibody to Histidine Rich Glycoprotein (HRG) Homo sapiens (Human) Polyclonal antibody

HPRG; HRGP; Histidine-Proline Rich Glycoprotein; Thrombophilia Due To Elevated HRG

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC534Hu01-Recombinant Histidine Rich Glycoprotein (HRG)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Histidine Rich Glycoprotein (HRG) Packages (Simulation)
  • Polyclonal Antibody to Histidine Rich Glycoprotein (HRG) Packages (Simulation)
  • Polyclonal Antibody to Histidine Rich Glycoprotein (HRG) DAB staining on IHC-P; Sample: Rat Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human HRG Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • PAC534Hu01.jpg Figure. Western Blot; Sample: Recombinant HRG, Human.
  • Polyclonal Antibody to Histidine Rich Glycoprotein (HRG) Western Blot; Sample: K562 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human HRG Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19) Selected
  • Polyclonal Antibody to Histidine Rich Glycoprotein (HRG) Knockout Varification:
    Lane 1: Wild-type K562 cell lysate;
    Lane 2: HRG knockout K562 cell lysate;
    Predicted MW: 59kd
    Observed MW: 55kd
    Primary Ab: 1µg/ml Rabbit Anti-Human HRG Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Histidine Rich Glycoprotein (HRG) Western Blot; Sample: Porcine Liver lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human HRG Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19) Selected
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HRG. It has been selected for its ability to recognize HRG in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MSACS: pr300330z
  • Evaluation of histidine-rich glycoprotein tissue RNA and serum protein as novel markers for breast cancerSpringer: Source
  • The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine‐rich glycoproteinPubMed: 25363753
  • Pharmacokinetic and Pharmacodynamic Investigations of ION-353382, a Model Antisense Oligonucleotide: Using Alpha-2-Macroglobulin and Murinoglobulin Double-Knockout MicePubmed:27031383
  • Compositions and Methods for Diagnosing Lung Cancery2016:0077095.html
  • Combination of serum histidine-rich glycoprotein and uterine artery Doppler to predict preeclampsiaPubmed:29416108
  • Ultrastructural Localization of Histidine-rich Glycoprotein in Skeletal Muscle Fibers: Colocalization With AMP DeaminasePubmed: 31880188
  • Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy …Pubmed: 32342301

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