Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) Homo sapiens (Human) Monoclonal antibody

HDAC1; GON10; HD1; RPD3; RPD3L1

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number E22
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPC206Hu01-Recombinant Histone Deacetylase 1 (HDAC1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) Packages (Simulation)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) Packages (Simulation)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 10ug/ml Mouse Anti-Human HDAC1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) DAB staining on IHC-P;
    Sample: Human Lung cancer Tissue;
    Primary Ab: 10ug/ml Mouse Anti-Human HDAC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) DAB staining on IHC-P;
    Sample: Rat Colon Tissue;
    Primary Ab: 10ug/ml Mouse Anti-Human HDAC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) DAB staining on IHC-P;
    Sample: Rat Testis Tissue;
    Primary Ab: 10ug/ml Mouse Anti-Human HDAC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) DAB staining on IHC-P;
    Sample: Mouse Pancreas Tissue;
    Primary Ab: 10ug/ml Mouse Anti-Human HDAC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) DAB staining on IHC-P;
    Sample: Mouse Cerebellum Tissue;
    Primary Ab: 10ug/ml Mouse Anti-Human HDAC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) FITC staining on IF;
    Sample: Human Hela cell;
    Primary Ab: 20ug/ml Mouse Anti-Human HDAC1 Antibody
    Second Ab: 4µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu18)
  • MAC206Hu22.jpg Western Blot; Sample: Lane1: Human Lung lysate; Lane2: A549 cell lysate; Lane3: HepG2 cell lysate; Lane4: Bovine Testis lysate; Lane5: Rat Testis lysate Primary Ab: 0.2μg/ml Mouse Anti-Human HDAC1 Antibody Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) Human Jurkat cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human HDAC1 Monoclonal Antibody (Catalog MAC206Hu22, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against HDAC1. It has been selected for its ability to recognize HDAC1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Flow cytometry: 10µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Bos taurus; Bovine (Cattle)

Giveaways

Citations

  • Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylasePubmed:25609369
  • Effects of tetrandrine and caffeine on cell viability and expression of mammalian target of rapamycin, phosphatase and tensin homolog, histone deacetylase 1, and histone acetyltransferase in glioma cellsScience: Article
  • Uridine protects against hypoxic-ischemic brain injury by reducing histone deacetylase activity in neonatal ratsPubMed: 26410212
  • Lacosamide reduces HDAC levels in the brain and improves memory: Potential for treatment of Alzheimer's diseasePubMed: 25931268
  • Effects of caffeine on cell viability and activity of histone deacetylase 1 and histone acetyltransferase in glioma cellsS1016319016300295

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