Polyclonal Antibody to High Temperature Requirement Factor A4 (HTRA4) Homo sapiens (Human) Polyclonal antibody

HtrA Serine Peptidase 4

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to High Temperature Requirement Factor A4 (HTRA4) Packages (Simulation)
  • Polyclonal Antibody to High Temperature Requirement Factor A4 (HTRA4) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HTRA4. It has been selected for its ability to recognize HTRA4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Upregulation of HtrA4 in the placentas of patients with severe pre-eclampsiaPubMed: 22964307
  • Human HtrA4 Expression Is Restricted to the Placenta, Is Significantly Up-Regulated in Early-Onset Preeclampsia, and High Levels of HtrA4 Cause Endothelial Dysfunction - See more at: http://press.endocrine.org/doi/abs/10.1210/jc.2014-3969#sthash.XcUOSx2Z.dpufPubMed: 25946029
  • HtrA3 Isoform-Specific ELISAs for Early Detection of Preeclampsia.pubmed:27932697

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