Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Homo sapiens (Human) Polyclonal antibody

G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB932Hu01-Recombinant Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Packages (Simulation)
  • Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Packages (Simulation)
  • Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human GAPDH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human GAPDH Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Figure: FITC staining on IHC-P;
    Sample: MCF7 cells.
  • PAB932Hu01.jpg Western Blot; Sample: Lane1: Bovine Liver lysate; Lane2: Bovine Cerebrum lysate; Lane3: Bovine Heart lysate; Lane4: Equine Liver lysate; Lane5: Equine Heart lysate Primary Ab: 0.5µg/mL Rabbit Anti-Human GAPDH Ab Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA
  • Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Western Blot; Sample: Human 293T cell lysate;
    Primary Ab: 0.25µg/ml Rabbit Anti-Human GAPDH Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Western Blot; Sample: Human Lymphocyte lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Human GAPDH Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Western Blot; Sample: Mouse Colon lysate;
    Primary Ab: 0.25µg/ml Rabbit Anti-Human GAPDH Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 0.25µg/ml Rabbit Anti-Human GAPDH Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Western Blot; Sample: Human A431 cell lysate;
    Primary Ab: 0.25µg/ml Rabbit Anti-Human GAPDH Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Equus caballus; Equine (Horse), Chicken (Gallus)

Giveaways

Citations

  • Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid LeukemiaPubMed: 26408402
  • Inactivation of the p53–KLF10–CEBPA Axis in Acute Myeloid LeukemiaPubMed: 26408402
  • Dapagliflozin modulates glucagon secretion in an SGLT2-independent manner in murine alpha cells.pubmed:28499695
  • Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egressionpubmed:28720868
  • Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipidsPubmed: 30237167
  • Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogelsPubmed: 30343256
  • Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood CellsPubmed: 30488202
  • Discovery, Optimization and Target Identification of Novel Potent Broad-Spectrum Antiviral InhibitorsPubmed: 30938999
  • Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathwayPubmed: 31078923
  • Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid LeukemiaPubmed: 31718075
  • Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor …Pubmed: 32046779
  • Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathwayPubmed: 32028241
  • Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in ratsPubmed: 33170419
  • Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo
  • Regulation of hepatic lipid metabolism in CB1-/-and Abcb4-/--HBV surface protein transgenic mice
  • BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 58133540760
  • Omega-3 Eicosapentaenoic Acid Reduces Prostate Tumor Vascularity33262291
  • Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026
  • Curcumin alters distinct molecular pathways in breast cancer subtypes revealed by integrated miRNA/mRNA expression analysisPubmed:34981672
  • Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegenerationPubmed:35151792
  • Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiencyPubmed:35577991

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