Polyclonal Antibody to Lipase, Endothelial (LIPG) Homo sapiens (Human) Polyclonal antibody

EL; EDL; Endothelial Lipase; Endothelial cell-derived lipase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA469Hu01-Recombinant Lipase, Endothelial (LIPG)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Lipase, Endothelial (LIPG) Packages (Simulation)
  • Polyclonal Antibody to Lipase, Endothelial (LIPG) Packages (Simulation)
  • Polyclonal Antibody to Lipase, Endothelial (LIPG) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human LIPG Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Lipase, Endothelial (LIPG) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human LIPG Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA469Hu01.jpg Figure. Western Blot; Sample: Recombinant LIPG, Human.
  • Polyclonal Antibody to Lipase, Endothelial (LIPG) Figure. Western Blot; Sample: Human Lung Tissue.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against LIPG. It has been selected for its ability to recognize LIPG in immunohistochemical staining and western blotting.

Usage

Western blotting: 1-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Lipase maturation factor 1 is required for endothelial lipase activityPubMed: 21447484
  • Randomized controlled study of the effect of a butter naturally enriched in trans fatty acids on blood lipids in healthy women1Ajcn: source
  • Effect of weight loss, independent of change in diet composition, on apolipoprotein AI kinetic in men with metabolic syndromePubmed: 23125458
  • Clinical efficacy of serum lipase subtype analy-sis for the differential diagnosis of pancreatic and non-pancreatic lipase elevationPubmed:27243230
  • Circulating C1q complement/TNF-related protein (CTRP) 1, CTRP9, CTRP12 and CTRP13 concentrations in Type 2 diabetes mellitus: In vivo regulation by glucose.pubmed:28207876
  • Association of plasma endothelial lipase levels on cognitive impairmentPubmed: 31216999
  • Treadmill Running Changes Endothelial Lipase Expression: Insights from Gene and Protein Analysis in Various Striated Muscle Tissues and Serum

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