• KO-Validated
Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC) Homo sapiens (Human) Polyclonal antibody

VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA145Hu01-Recombinant Vascular Endothelial Growth Factor C (VEGFC)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC) Packages (Simulation)
  • Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC) Packages (Simulation)
  • Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC) Used in DAB staining on fromalin fixed paraffin-embedded Bowels tissue
  • PAA145Hu01.jpg Figure. Western Blot; Sample: Recombinant VEGFC, Human.
  • Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC) Western Blot; Sample: Mouse raw264.7 cell lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Human VEGFC Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC) Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: VEGFC knockout MCF7 cell lysate;
    Predicted MW: 47kd
    Observed MW: 44kd
    Primary Ab: 3µg/ml Rabbit Anti-Human VEGFC Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Vascular Endothelial Growth Factor C (VEGFC) Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Human VEGFC Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against VEGFC. It has been selected for its ability to recognize VEGFC in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Citations

  • Alternatively activated RAW264. 7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinomaPubMed: 19241443
  • Expression of COX-2 and VEGF-C in cholangiocarcinomas at different clinical and pathological stagesPubMed: 26125824
  • Fenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxiaPubMed: 26622498
  • IL-10 Indirectly Regulates Corneal Lymphangiogenesis and Resolution of Inflammation via MacrophagesPubMed: 26608451
  • Interleukin-6 Induces Vascular Endothelial Growth Factor-C Expression via Src-FAK-STAT3 Signaling in Lymphatic Endothelial Cells.pubmed:27383632
  • RNAi-mediated gene silencing of vascular endothelial growth factor C suppresses growth andinduces apoptosis in mouse breast cancer in vitro and in vivo.pubmed:27895746
  • Characterization of isolated liver sinusoidal endothelial cells for liver bioengineeringPubmed:29582235
  • Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cellsPubmed:29328465
  • Dynamic signature of lymphangiogenesis during acute kidney injury and chronic kidney diseasePubmed: 31019289
  • Isotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions?Pubmed: 32722957

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