Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) Homo sapiens (Human) Polyclonal antibody

HGPRT; HGPRTase; HPRT; Lesch-Nyhan Syndrome; Hypoxanthine-guanine phosphoribosyltransferase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA717Hu01-Recombinant Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) Packages (Simulation)
  • Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) Packages (Simulation)
  • Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human HPR
  • Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) DAB staining on IHC-P;
    Samples: Human Liver Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human HPRT1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human HPRT1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human HPRT1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) DAB staining on IHC-P;
    Samples: Human Breast cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human HPRT1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) DAB staining on IHC-P;
    Samples: Human Prostate Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human HPRT1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA717Hu01.jpg Figure. Western Blot; Sample: Recombinant HPRT1, Human.
  • Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) Figure. Western Blot; Sample: Lane1: Rat Liver Tissue; Lane2: Rat Brain Tissue; Lane3: Human Hela Cells; Lane4: Human HepG2 Cells; Lane5: Human A549 Cells.
  • Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human HPRT1 Polyclonal Antibody (Catalog # PAA717Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HPRT1. It has been selected for its ability to recognize HPRT1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-5µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Citations

  • Response to infliximab therapy in ulcerative colitis is associated with decreased monocyte activation, reduced CCL2 expression and downregulation of Tenascin C.Pubmed:25518051
  • Response to infliximab therapy in ulcerative colitis is associated with decreased monocyte activation, reduced CCL2 expression and downregulation of tenascin CPubMed: 25518051

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