Polyclonal Antibody to Immunoglobulin G4 (IgG4) Homo sapiens (Human) Polyclonal antibody

IGHG4; Ig Gamma-4 Chain C Region; Immunoglobulin Heavy Constant Gamma 4; G4m Marker; Immunoglobulin Gm4

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen NPA234Hu01-Native Immunoglobulin G4 (IgG4)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Immunoglobulin G4 (IgG4) Packages (Simulation)
  • Polyclonal Antibody to Immunoglobulin G4 (IgG4) Packages (Simulation)
  • Polyclonal Antibody to Immunoglobulin G4 (IgG4) DAB staining on IHC-P; Samples: Human Tonsil Tissue; Primary Ab: 10ug/ml Rabbit Anti-Human IgG4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Immunoglobulin G4 (IgG4) DAB staining on IHC-P;
    Sample: Human Spleen Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human IgG4 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
    Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Immunoglobulin G4 (IgG4) DAB staining on IHC-P;
    Sample: Mouse Stomach Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human IgG4 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
    Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Immunoglobulin G4 (IgG4) DAB staining on IHC-P;
    Sample: Mouse Spleen Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human IgG4 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA234Hu01.jpg Western Blot; Sample: Human Serum Primary Ab: 0.005µg/ml Rabbit Anti-Human IgG4 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
  • Polyclonal Antibody to Immunoglobulin G4 (IgG4) Western Blot; Sample: Porcine Serum
    Primary Ab: 0.05µg/ml Rabbit Anti-Human IgG4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19) Selected
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IgG4. It has been selected for its ability to recognize IgG4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.005-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingHindawi:Source
  • Utility of serum IgG, IgG4 and carbonic anhydrase II antibodies in distinguishing autoimmune pancreatitis from pancreatic cancer and chronic pancreatitisPubmed:25194335
  • Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancerPubmed:25529861
  • Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2.0 CellsPubmed:25561924
  • Loss of interleukin-21 leads to atrophic germinal centers in multicentric Castleman’s diseasePubMed: 26377996
  • The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingPubMed: 26161394
  • Increasing Foxp3, IgG4 and improvement quality of life after immunotherapy treatment in allergic rhinitis patientsarticle:30785

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