Monoclonal Antibody to Insulin Degrading Enzyme (IDE) Homo sapiens (Human) Monoclonal antibody

Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C2
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB897Hu01-Recombinant Insulin Degrading Enzyme (IDE)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.8mg/mL
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  • Monoclonal Antibody to Insulin Degrading Enzyme (IDE) Packages (Simulation)
  • Monoclonal Antibody to Insulin Degrading Enzyme (IDE) Packages (Simulation)
  • MAB897Hu22.jpg Western Blot; Sample: K562 cell lysate Primary Ab: 0.2µg/ml Mouse Anti-Human IDE Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against IDE. It has been selected for its ability to recognize IDE in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • ReductionPubMed: 26442993
  • Peptide hydroxamate derivatives as regulators for insulin receptor signaling and its degradation by zinc metalloprotease in diabetic ratsarticle-full-text-pdf
  • Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats.pubmed:27930980
  • Nigella sativa Oil and Chromium Picolinate Ameliorate Fructose-Induced Hyperinsulinemia by Enhancing Insulin Signaling and Suppressing Insulin-Degrading Enzyme in Male Rats10.1007/s12011-017-1167-z
  • The Protective Effects of IGF-I against β-Amyloid-related Downregulation of Hippocampal Somatostatinergic System Involve Activation of Akt and Protein Kinase APubmed:29406271

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