Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1)

IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 113.00 US$ 265.00 US$ 378.00 US$ 945.00 US$ 3,780.00
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Overview

Properties

Product No.PAA052Bo01
Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryTumor immunity Endocrinology Cardiovascular biology

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA052Bo01-Recombinant Insulin Like Growth Factor Binding Protein 1 (IGFBP1)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Bovine Testis Tissue; Primary Ab: 20µg/ml Rabbit Anti-Bovine IGFBP1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant IGFBP1, Cattle.
Western Blot; Sample: HepG2 cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Bovine IGFBP1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Western Blot; Sample: Bovine Serum;
Primary Ab: 3µg/ml Rabbit Anti-Bovine IGFBP1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IGFBP1. It has been selected for its ability to recognize IGFBP1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

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Citations

Lack of Dietary Carbohydrates Induces Hepatic Growth Hormone (GH) Resistance in RatsEndo: source
Serum IGF-I Is Not a Reliable Pharmacodynamic Marker of Exogenous Growth Hormone Activity in MiceEndo: source
Effects of a dietary crude fibre concentrate on growth in weaned piglets.pubmed:28318464
Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cellspubmed:29096715