Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 2 (IGFBP2) Sus scrofa; Porcine (Pig) Polyclonal antibody

IBP2; IGF-BP53

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA053Po01-Recombinant Insulin Like Growth Factor Binding Protein 2 (IGFBP2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 2 (IGFBP2) Packages (Simulation)
  • Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 2 (IGFBP2) Packages (Simulation)
  • Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 2 (IGFBP2) DAB staining on IHC-P; Samples: Porcine Cardiac Muscle Tissue; Primary Ab: 20µg/ml Rabbit Anti-Porcine IGFBP2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 2 (IGFBP2) DAB staining on IHC-P;
    Samples: Porcine Liver Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Porcine IGFBP2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 2 (IGFBP2) DAB staining on IHC-P;
    Samples: Porcine Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Porcine IGFBP2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 2 (IGFBP2) DAB staining on IHC-P;
    Sample: Mouse Cerebrum Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GFAP Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 2 (IGFBP2) DAB staining on IHC-P;
    Sample: Porcine Cardiac Muscle Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Porcine IGFBP2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 2 (IGFBP2) DAB staining on IHC-P;
    Sample: Porcine Liver Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Porcine IGFBP2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 2 (IGFBP2) DAB staining on IHC-P;
    Sample: Porcine Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Porcine IGFBP2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA053Po01.jpg Western Blot; Sample: Porcine Serum Primary Ab: 0.3µg/ml Rabbit Anti-Porcine IGFBP2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IGFBP2. It has been selected for its ability to recognize IGFBP2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • A combined biomarker panel shows improved sensitivity for the early detection of ovarian cancer allowing the identification of the most aggressive type II tumours.pubmed:28664912
  • Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D‑LC‑MS/MSDoi: 10.3892/etm.2018.6818
  • Serum IGFBP2 Level Is a New Candidate Biomarker of Severe Malnutrition in Advanced Lung CancerPubmed: 32286106

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