Monoclonal Antibody to Insulin Receptor (INSR) Homo sapiens (Human) Monoclonal antibody

CD220; ISR; HHF5; IR

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2a Kappa, Clone Number C1
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA895Hu02-Recombinant Insulin Receptor (INSR)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.94mg/ml
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  • Monoclonal Antibody to Insulin Receptor (INSR) Packages (Simulation)
  • Monoclonal Antibody to Insulin Receptor (INSR) Packages (Simulation)
  • Monoclonal Antibody to Insulin Receptor (INSR) FITC staining on IF; Samples: Human MCF7 cell; Primary Ab: 20μg/ml Mouse Anti-Human ISR Antibody Second Ab: 5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
  • Monoclonal Antibody to Insulin Receptor (INSR) AF488 staining on IF;
    Sample: MCF7 cell
    Primary Ab: 30µg/ml Mouse Anti-Human INSR Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • MAA895Hu22.jpg Western Blot; Sample: Lane1: MCF7 cell lysate; Lane2: HCT116 cell lysate Primary Ab: 0.8µg/ml Mouse Anti-Human INSR Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against INSR. It has been selected for its ability to recognize INSR in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Molecular and Biochemical Effect of Neem Extract On Experimental Diabetes Org:Source
  • Age-Dependent Onset of Insulin Resistance in Insulin-Resistant MicePubMed: 26632184
  • A newly developed silymarin nanoformulation as a potential antidiabetic agent in experimental diabetespubmed:27623396
  • Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β.pubmed:28337257
  • Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats.pubmed:27930980
  • Regulation of insulin receptor phosphorylation in the brains of prenatally stressed rats: New insight into the benefits of antidepressant drug treatment.pubmed:28063625
  • (台灣農業研究67 (4): 355-364) 香菇柄水萃物改善糖尿病降低阿茲海默氏症罹病風險初探
  • Mechanism underlying starvation-dependent modulation of olfactory behavior in Drosophila larvaPubmed: 32080342
  • Prenatal Stress Induced Spatial Memory Deficit in a Sex-Specific Manner in Mice: Possible Involvement of Hippocampal Insulin Resistance
  • Internal State Affects Local Neuron Function in an Early Sensory Processing Center to Shape Olfactory Behavior in Drosophila Larvae

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